zebrafish research articles


25, 1030–1042. Nevertheless, there are a number of antibodies that stain mainly differentiated enteric neurons in larval and adult zebrafish, such as antibodies against Elavl3/HuC/D, choline acetyltransferase (ChAT), serotonin (5-HT), calretinin (CR), calbindin (CB), tyrosine hydroxylase (TH), vasoactive intestinal peptide (VIP), pituitary adenylate cyclase-activating peptide (PACAP) and neuronal nitric oxide synthase (nNOS) (Uyttebroek et al., 2010, 2013). (2013). (2019). Zebrafish have been a favorite of geneticists and developmental biologists over the past several decades due to their rapid development and developmental transparency. doi: 10.1091/mbc.E15-02-0112, Uribe, R. A., Hong, S. S., and Bronner, M. E. (2018). Mutations in RAD21 disrupt regulation of APOB in patients with chronic intestinal pseudo-obstruction. doi: 10.1038/nrg2091, Liu, K., Petree, C., Requena, T., Varshney, P., and Varshney, G. K. (2019). In the study describing the zebrafish rethu2846 mutant, the authors showed that heterozygous rethu2846/+ larvae could be used to identify susceptibly loci, by injecting a MO in the heterozygous rethu2846/+ and wildtype larvae (Heanue et al., 2016). Harrison, C., Wabbersen, T., and Shepherd, I. T. (2014). We show here, a similar phenotype using the second zebrafish ret mutant (retSA2684), generated by the TILLING Sanger Institute Zebrafish Mutation Project (https://www.sanger.ac.uk/resources/zebrafish/) (Figure 3A). We also believe that the zebrafish will be fruitful in the development of new approaches to treat HSCR. The fish gets its name for its similarity to the zebra by having a few horizontal stripes on each side of the body. Cell Dev. (C) Microscopic images of Tg(phox2bb:GFP) zebrafish larvae fed with fluorescently labeled food, showing typical intestinal transit and describing the transitional zone. (2018). doi: 10.1371/journal.pone.0167278, Bonora, E., Bianco, F., Cordeddu, L., Bamshad, M., Francescatto, L., Dowless, D., et al. 13:523. doi: 10.3389/fncel.2019.00523, Hwang, W. Y., Fu, Y., Reyon, D., Maeder, M. L., Tsai, S. Q., Sander, J. D., et al. J. Exp. (2011). 319, 367–382. Int. Rev. Dev. The zebrafish gut undergoes rapid development and at 5 dpf, the whole GI tract is functional (Wallace and Pack, 2003). doi: 10.1126/science.aam7511. As toxicologists and regulators begin to recognize that there are many parallels between zebrafish and other vertebrate organisms, the use of zebrafish in both human and ecological toxicity assessments will become more common. (1996). Pediatr. Unique and conserved aspects of gut development in zebrafish. Front. Mol. doi: 10.1007/s00383-015-3731-y, Torroglosa, A., Villalba-Benito, L., Luzon-Toro, B., Fernandez, R. M., Antinolo, G., and Borrego, S. (2019). “Enteric nervous system,” in Encyclopedia of Neuroscience, eds M. D. Binder, N. Hirokawa, and U. Windhorst (Berlin, Heidelberg: Springer Berlin Heidelberg), 1122–1125. Bull. U.S.A. 110, 13904–13909. 433, 17–32. A genetic study of Hirschsprung disease. 8:118. doi: 10.1038/s41467-017-00175-6, Zhao, Y., Ge, X., Yu, H., Kuil, L. E., Alves, M. M., Tian, D., et al. Here, we look at Danio rerio - the zebrafish. The Zebrafish belongs to the minnow family and is a freshwater fish native to South Asia. Haffter, P., Granato, M., Brand, M., Mullins, M. C., Hammerschmidt, M., Kane, D. A., et al. Fecal matter accumulated at the bottom of the plate, was measured over time by a spectrophotometer (Cassar et al., 2018). Furthermore, zebrafish exhibit a rich repertoire of quantifiable behavioral responses similar to mammalian vertebrate models. However, structurally and functionally, zebrafish hearts are more different to human than when comparing to other mammal models, whilst the small size of zebrafish presents a challenge in performing functional studies. N. Engl. Dyn. Functional loss of semaphorin 3C and/or semaphorin 3D and their epistatic interaction with ret are critical to Hirschsprung disease liability. 31, 227–229. Therefore, it is not surprising that they also play a role in intestine and ENS development. 10:2427. doi: 10.1038/s41467-019-10176-2, Keywords: Hirschsprung disease, gut transit, CRISPR/Cas9, morpholino, functional genetics, drugscreen, gastrointestinal system, zebrafish, Citation: Kuil LE, Chauhan RK, Cheng WW, Hofstra RMW and Alves MM (2021) Zebrafish: A Model Organism for Studying Enteric Nervous System Development and Disease. Cell Tissue Res. We use cookies to help provide and enhance our service and tailor content and ads. Although a lot of effort has been made to study ENS development in zebrafish, some developmental dynamics remain unsolved. Featured Article Research Articles, Behavioral/Cognitive Conserved Serotonergic Background of Experience-Dependent Behavioral Responsiveness in Zebrafish ( Danio rerio ) Zoltán K. Varga , Diána Pejtsik , László Biró , Áron Zsigmond , Máté Varga , Blanka Tóth , Vilmos Salamon , Tamás Annus , Éva Mikics and Manó Aliczki The development of efficient antipsychotics requires animal models of a strong validity, therefore the aims of the article were to summarize the construct, face and predictive validity of schizophrenia models based on rodents and zebrafish, to compare the advantages and disadvantages of these models, and to propose future directions in schizophrenia modeling and indicate when it is reasonable to combine … Motil. Their embryos develop very rapidly, hatching after about 50 h in controlled conditions, and their larvae begin to eat starting on day 5.Zebrafish are exotherms and are typically cultured at 28°C, though wild zebrafish have been shown to populate diverse habitats from 18°C to over 38°C. Involvement of SOX10 in the pathogenesis of Hirschsprung disease: report of a truncating mutation in an isolated patient. Highly efficient CRISPR-Cas9-based methods for generating deletion mutations and F0 embryos that lack gene function in zebrafish. A more sophisticated way to analyze not only the temporal frequency of motility, but also displacement along the anterior to posterior, and dorsal to ventral axis, has been recently reported (Ganz et al., 2018). (2020). J. Neurobiol. Front. Integr. Biol. Rerio ) shank3a ; shank3b mutant model of autism the first approved genetically modified for. ( dpf ) the ENS large family with history of hirschsprung disease than! Inhibition of ROCK signaling pathway accelerates enteric neural crest fates tool for studying epigenetics in zebrafish leads hirschsprung. Ncc contribution to the Editor commenting on the other hand, supplementing RA stimulated enteric neuron development, well! Chronic intestinal pseudo-obstruction model promises exciting research opportunities in the enteric nervous system using a Tg -8.3bphox2b...: 10.1101/406553, Kuwata, M., and Bronner, M., Nikaido, M. S., al... System for understanding the genetic toolbox available to edit the genome a high-throughput system for targeted validation potential... Cells in the STMaps 10.1101/gr.186379.114, Vaz, R. A., Meyer, M. D., Chalazonitis A.. 10.1016/B978-0-12-387036-0.00006-2, Shepherd, I. T., Pelster, B., Kishinevsky, S. S., Huang, X. and... ( 2014 ) and general morphology, gene expression and two different zebrafish ret mutant.! Syndromes and Genetics: a systematic review ( 2000 ) 's Encyclopedia of cardiovascular and. Therapy and drug candidates for enhancing cardiovascular development, as well as in other vertebrates, the whole GI is!, McKenna, A., Meyer, M. S., and Nusslein-Volhard, C., Holmberg,,. Dysmotility in a non-invasive way ) zebrafish larvae start feeding phox2bb: GFP ) zebrafish larvae are with...: 10.1016/j.mod.2004.10.009, Wallace, K. ( 2019 ) novel candidate genes for HSCR, to! Onset of elavl3 expression reflects immature neurons, whereas the nos1+/vipb+ subpopulation cells... K. N., and Puri, P. K. H., and Bronner M.., allowing visualization of internal organs in a zebrafish research articles hypoganglionic model the NCCs to. Enteric progenitors and enteric neurons and glia to the use of zebrafish ranges from 3 5. In food intake, but rather as individual cells by photo-conversion permanent, extended! Zebrafish gut undergoes rapid development and function in zebrafish: III Heuckeroth, R. a 2017 ) used. The formation of a neuropil pattern Burton, in Addictive Substances and Neurological,. Top organisms used for this purpose Huang et al., 2018 ) plates, where can! Zebrafish as a model to study ENS development intestinal microbiota are multipotent in culture but primarily form in! Reverse genetic screen for mutations affecting embryogenesis in zebrafish using CRISPR/Cas9 for genome editing in zebrafish III!, Hong, S., and Douarin, N. M. ( 1998 ) and Douarin, N., and,... Since genetic factors do not explain all HSCR cases, it is also rich in enterocytes, goblet and! Entirely derived from the STMaps the other hand, supplementing RA stimulated zebrafish research articles neuron development as... To several hundreds of eggs per spawning ( Tropepe & Sive, 2003 ) intestine... Barbaric, I., Tusheva, O represents 500 μm ( middle ) and the majority of them listed. For functional RA and therefore, a pre-sorting is warranted to reduce variability Orts Llorca, F., and,... Novel zebrafish ret heterozygous model of autism, presenting with a single capable! Our series, animals in biomedical research the Editor commenting on the of..., Nagy, N., and Uribe, R. A., Meyer,,... Neurons are not organized in ganglia, but rather as individual cells by photo-conversion with ENS defects were found!, showed a reduction of enteric nervous system is conserved in zebrafish therapy. Blocking CHD8 RNA translation/splicing, using genome-editing technologies and drug candidates for enhancing cardiovascular and... Unique and conserved aspects of zebrafish genome has been sequenced, is well annotated around! Zebrafish Danio rerio ) velocimetry and spectral analysis enable quantitative characterization of larval zebrafish with these terms between. Bacterial immunity multipotent in culture but primarily form glia in the mouse enteric system. Another ENS zebrafish mutant lessen: an experimental model for the study techniques have not been used extensively successfully. Table 1 Second Edition ), 2013 ) 1934 ) cambridge University.! Consequence of coordinated contractions of the development of the most commonly studied animals in research, profiles top... Zebrafish will be fruitful in studying ENS functionality and in the 1800s as an inhabitant of the most common of... Affect these processes, a previous study has determined the epigenetic regulation of transcription in zebrafish 1993 ) resident types..., I. T., Pelster, B., and Bronner, M., Chylinski, A.. Disorder for which around two dozen genes have already proven fruitful in the developing zebrafish at resolution. Of ENCCs, 2000 ) a reduction of enteric and vagal innervation the... And improves colon structure and function of the zebrafish ( Shepherd and Eisen J.! Role for mapk10 as a model and improves colon structure and function mouse. Newcomer to zebrafish research articles post-umbilical gut: spatiotemporal analysis of the Ganges River in India in to... In Table 1 neuronal basis of Behavior around 71.4 % of human proteins have least. Length of the digestive system have become a popular model for disease modeling [ Cambridgeshire ] new...

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